In previous blog we discussed about what is Salesforce1 User, user with Salesforce1 checkbox ticked will access Salesforce1 from supported web browser from supported device.
When we untick Enable the Salesforce1 mobile browser app in Salesforce1 setting,
Salesforce1 User option in user detail will be removed.
But, on top of that, when you disable above setting, you will also no longer able to use Salesforce1 with simulator:
- one/one.app , example https://na3.salesforce.com/one/one.app, or
- extention/app for Google Chrome
You will get error message URL No Longer Exists
Reference: Salesforce1 Simulator using one/one.app